Table 5 Primer sequences for virulence genes of K. pneumoniae
Toxicity gene types | Gene name | Primer sequence (5 ‘-3’) | Fragment size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
Pili | fimH | F: GTCTACGTTAACCTGACCCCG R: ATTGATAGACAAAGGTGATGCCGAT | 781 | 56 |
mrkD | F: AAGCTATCGCTGTACTTCCGGCA R: GGCGTTGGCGCTCAGATAGG | 340 | 60 | |
lipopolysaccharide | uge | F: TCTTCACGCCTTCCTTCACT R: GATCATCCGGTCTCCCTGT | 534 | 53 |
wabG | F: CTCTGGTGCGGCAGAAGTAC R: TGGCCGTCGACGATAAACTC | 931 | 52 | |
Capsule | rmpA | F: ACCCTTTACAGCCAAATTTTCTTGT R: CTGGGCTACCTCTGCTTCATAT | 468 | 52 |
magA | F: TGATAAGTGGCGGAGATTCTGA R: TGATAAGTGGCGGAGATTCTGA | 542 | 52 | |
iroN | F: GAATGAAACTACCGCCCCCA R: TGTGGAGTGGAGGCGAGATA | 1 033 | 54 | |
Iron carrier | entB | F: ATTTCCTCAACTTCTGGGGC R: AGCATCGGTGGCGGTGGTCA | 371 | 60 |
iucA | F: GCTTATTTCTCCCCAACCC R: TCAGCCCTTTAGCGACAAG | 583 | 59 | |
kfu | F: GAAGTGACGCTGTTTCTGGC R: TTTCGTGTGGCCAGTGACTC | 797 | 55 | |
Allantoin | allS | F: CTTCAGCAGATAAATGACGGGGTAG R: GTGGGTAAACCGCCATATTTTCC | 244 | 56 |
urease | ureA | F: GCTGACTTAAGAGAACGTTATG R: GATCATGGCGCTACCT(C/T)A | 337 | 55 |