Table 4 Primer sequences for resistance genes of K. pneumoniae
Types of antibacterial drugs | Gene name | Primer sequence (5 ‘-3’) | Fragment size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
β - lactams | blaTEM | F: TCGCCGCATACACTATTCTCAGAATGA R: ACGCTCACCGGCTCCAGATTTAT | 445 | 55 |
blaCTX | F: ACTTCAGCCACACGGATTCA R: AAGTGGAGCGACAGAGC | 905 | 57 | |
blaNDM | F: GGTTTGGCGATCTGGTTTTC R: CGGAATGGCTCATCACGATC | 621 | 55 | |
blaOXA−48 | F: GCGTGGTTAAGGATGAACAC R: CATCAAGTTCAACCCAACCG | 438 | 55 | |
blaIMP | F: GGAATAGAGTGGCTTAAYTCTC R: GGTTTAAYAAAACAACCACC | 232 | 55 | |
blaVIM | F: GATGGTGTTTGGTCGCATA R: CGAATGCGCAGCACCAG | 390 | 55 | |
blaDHA | F: GCCTGTTTGGTGCTCTGA R: GCACGGTTATACGGCTGA | 460 | 55 | |
blaKPC | F: CGTCTAGTTCTGCTGTCTTG R: CTTGTCATCCTTGTTAGGCG | 798 | 55 | |
Aminoglycosides | aadA | F: GTGGATGGCGGCCTGAAGCC R: AATGCCCAGTCGGCAGCG | 525 | 62 |
Tetracyclines | tet(B) | F: AAACCATTACGGCATTCTGC R: GACCGGATACACCATCCATC | 659 | 55 |
Chloramphenicol class | cmlA | F: GGCCTCGCTCTTACGTCATC R: GCGACACCAATACCCACTAGC | 989 | 57 |
floR | F: TATCTCCCTGTCGTTCCAG R: AGAACTCGCCGATCAATG | 399 | 53 | |
Sulfonamide class | sul2 | F: CGGCATCGTCAACATAACCT R: TGTGCGGATGAAGTCAGCTC | 721 | 66 |
Quinolones | parC | F: CTGGGTAAATACCATCCGCAC R: CGGTTCATCTTCATTACGAA | 987 | 59 |
gyrA | F: ATGAGCGACCCTTGCGAGAGAAAT R: AGCCCTTCAATGCTGATGTCTTC | 685 | 60 | |
gyrB | F: CCTCCCAGACCAAAGACAAACT R: CAGCATTGCTTTCGGATAACG | 821 | 53 |