Table 2 K. pneumoniae typing identification primer sequence
Gene name | Primer sequence (5 ‘-3’) | Fragment size (bp) | Annealing temperature (°C) |
|---|---|---|---|
ropB | F: GGCGAAATGGCWGAGAACCA R: GAGTCTTCGAAGTTGTAACC | 1075 | 50 |
gapA | F: TGAAATATGACTCCACTCACGG R: CTTCAGAAGCGGCTTTGATGGCTT | 662 | 60 |
mdh | F: CCCAACTCGCTTCAGGTTCAG R: CCGTTTTTCCCCAGCAGCAG | 756 | 50 |
pgi | F: GAGAAAAACCTGCCTGTACTGCTGGC R: CGCGCCACGCTTTATAGCGGTTAAT | 566 | 50 |
phoE | F: ACCTACCGCAACACCGACTTCTTCGG R: TGATCAGAACTGGTAGGTGAT | 602 | 50 |
infB | F: CTCGCTGCTGGACTATATTCG R: CGCTTTCAGCTCAAGAACTTC | 462 | 50 |
tonB | F: CTTTATACCTCGGTACATCAGGTT R: ATTCGCCGGCTGRGCRGAGAG | 539 | 45 |
wzi | F: GTGCCGCGAGCGCTTTCTATCTTGGTATTCC R: GAGAGCCACTGGTTCCAGAACTTGACCGC | 580 | 60 |