Table 3 Primers used for real-time PCR in this study
Items | Nucleotide sequence of primers (5’-3’) | Product size, bp | GenBank Accession | Annealing temperature, °C |
|---|---|---|---|---|
SGLT-1 (SLC5A) | Forward: TCATCATCGTCCTGGTCGTCTC Reverse: CTTCTGGGGCTTCTTGAATGTC | 144 | XM_021072101.1 | 58 |
GLUT2 (SLC2A2) | Forward: GCACATCCTGCTTGGTCTATCT Reverse: CACTTGATGCTTCTTCCCTTTC | 203 | NM_001097417.1 | 60.5 |
GLUT4 (SLC2A4) | Forward: AGGCACCCTCACTACCCTCT Reverse: CTTCTTCCTTCCCAGCCACT | 109 | NM_001128433.1 | 60 |
T1R2 | Forward: TCGCCTCGTGCTGTCATAG Reverse: CCACATCTCAGAGCCTGACC | 317 | XM_021093444.1 | 60 |
T1R3 | Forward: TGTACCAGGTTCTCGTCCCT Reverse: GGCCATGAACACTAGGCTG | 172 | NM_001113288.1 | 60 |
β-actin | Forward: CATCGTCCACCGCAAAT Reverse: TGTCACCTTCACCGTTCC | 210 | NC_010445 | 60 |