Table 2 Real-time PCR primers and amplified PCR product size for piglets
Gene | Primer sequence (5′- 3′) | Serial number | PCR Products size (bp) |
|---|---|---|---|
GLUT2 | F: ATTGTCACAGGCATTCTTGTTAGTCA | NM_001097417.1 | 273 |
R: TTCACTTGATGCTTCTTCCCTTTC | |||
SGLT1 | F: TCATCATCGTCCTGGTCGTCTC | NM_001164021.1 | 144 |
R: CTTCTGGGGCTTCTTGAATGTC | |||
SLC1A1 | F: GGCACCGCACTCTACGAAGCA | NM_001164649.1 | 177 |
R: GCCCACGGCACTTAGCACGA | |||
SLC7A1 | F: TCTGGTCCTGGGCTTCATAA | XM_021065165.1 | 123 |
R: ACCTTCGTGGCATTGTTCAG | |||
SLC7A7 | F: CTCGGGCATCTTCGTCT | XM_013978228.2 | 126 |
R: CCCAGTTCCGCATAACA | |||
SLC38A2 | F: GTTACCTTTGGTGATCCAGGC | XM_013997964.2 | 96 |
R: ACCAATGACACCAGCAGAACC | |||
PEPT1 | F: CAGACTTCGACCACAACGGA | NM_214347.1 | 99 |
R: TTATCCCGCCAGTACCCAGA | |||
FABP4 | F: TGGAAACTTGTCTCCAGTG | NM_001002817.1 | 147 |
R: GGTACTTTCTGATCTAATGGTG | |||
FATP-1 | F: GGAGTAGAGGGCAAAGCAGG | XM_021076151.1 | 208 |
R: AGGTCTGGCGTGGGTCAAAG | |||
ACTβ | F: TGGAACGGTGAAGGTGACAGC | XM_003124280.5 | 177 |
R: GCTTTTGGGAAGGCAGGGACT |