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Table 2 Group-specific oligonucleotides for qPCR classification of IgM protease

From: Serotype independent protection induced by a vaccine based on the IgM protease of Streptococcus suis and proposal for a new immunity-based classification system

Primer mix

Primer names

Sequences

Group A - forward

ideS-A1

ideS-A2

gtg gta ttg agt ctg gtt gtt c

gtc aga cac cct aat cgt ag

Group A - reverse

ideS-A3

ideS-A4

gtt tca act aac tct gtc tc

atg agc tat cag aat act gtg

Group B - forward

ideS-B1a

ideS-B1b

ideS-B2

agt ttc ttc caa cat gtt gc

agt ttc ttc caa tat gtt gc

atc agc tcc ata ttt ctc

Group B - reverse

ideS-B3

ideS-B4a

ideS-B4b

gtg aaa aag taa cca ata ac

tat tta ata gcc ttt tct ct

tat tta ata gct ttt tct ct

Group C - forward

ideS-C1

gca act tga tct tct tct ttt ac

Group C - reverse

ideS-C2

gaa ctt cgt gaa tca gaa ac