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Table 4 Slopes (Beta), Confidence Intervals (CIs) and p-value of the factors (swIAV subtype, stabilizers, time after spiking, and temperature) influencing Ct-values usinga multivariable linear analysis (N = NucleoProtect VET Reagent®, C = Control, P = Primestore®, V = Sigma-Virocult®)

From: Effect of stabilizers on the detection of swine influenza A virus (swIAV) in spiked oral fluids over time

Independent variable

Multivariable analysis

Beta

95% CI

p-value

swIAV subtype

   

H1avN2–H1pdmN2

2.26

1.27, 3.25

 < 0.001

Stabilizers

   

C–N

− 5.98

− 8.27, − 3.69

 < 0.001

C–P

− 5.22

− 7.51, − 2.94

 < 0.001

N–P

0.76

− 1.53, 3.05

0.8

C–V

− 6.09

− 8.38, − 3.80

 < 0.001

N–V

− 0.11

− 2.40, 2.18

 > 0.9

P–V

− 0.86

− 3.15, 1.43

0.8

Time after spiking

   

72–168 h

− 0.04

− 1.50, 1.42

0.998

72–336 h

1.35

− 0.10, 2.81

0.074

168–336 h

1.39

− 0.07, 2.85

0.065

Temperature

   

4–22 °C

2.53

1.54, 3.52

 < 0.001

  1. Bold values represent significant results